Research Use Only. Peptides Lab UK supplies research-grade materials for in-vitro laboratory research only. Nothing on this page is medical advice or an instruction for human or animal use, and no product sold on this website is a medicine.
What this page covers
Laboratory protocols often require a lyophilised peptide to be reconstituted to a known concentration. This page explains the arithmetic only: converting between mass, volume and concentration. It does not give, recommend or imply any dose for humans or animals.
The core formula
Concentration (mg/mL) = mass of peptide in the vial (mg) ÷ volume of diluent added (mL).
Worked example: a vial containing 5 mg of lyophilised peptide reconstituted with 2.0 mL of diluent gives 5 ÷ 2.0 = 2.5 mg/mL, which is 2,500 µg/mL.
Converting a required mass to a volume
Volume (mL) = required mass (mg) ÷ concentration (mg/mL). For example, to obtain 0.5 mg of peptide for an in-vitro assay from a 2.5 mg/mL solution: 0.5 ÷ 2.5 = 0.2 mL. Remember 1 mg = 1,000 µg.
Graduation scales
Some laboratory syringes and pipettes are graduated on a 100-unit scale where 100 units equal 1.0 mL, so 1 unit = 0.01 mL. Converting 0.2 mL on such a scale gives 20 units. This is a volume conversion only.
Good laboratory practice
- Record lot number, vial mass, diluent and volume.
- Use calibrated volumetric equipment and aseptic technique.
- Verify the stated vial content against the certificate of analysis (HPLC/MS).
- Store solutions as specified and within a validated stability window.
Use our peptide calculator to check concentration arithmetic.
UK regulatory status
In the UK, medicines are regulated by the Medicines and Healthcare products Regulatory Agency (MHRA). Research-grade compounds sold by Peptides Lab UK are supplied strictly for laboratory research; they are not medicinal products, are not manufactured to medicinal GMP standards and must not be used in humans or animals.
Related research pages
Disclaimer: All products sold by Peptides Lab UK are for laboratory research use only. Not for human or veterinary use, not for consumption, and not intended to diagnose, treat, cure or prevent any disease. This page is an educational literature summary and is not medical advice.
